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Notice the pink discoloration giving Betty's dress a purple hue. The original blue color shows at the waistline and lower bodice. A large section was cut from the lower right. In the photo on the right, you can see the torn lining and the upper part of the zipper. The dress was relatively new when the Hills' event occurred but was damaged after they returned home late, having seen a silent, hovering anomalous object only 100 feet overhead.
In a 2020 television show, host Ben Hansen and Kathleen Marden had the opportunity to visit the Milne Special Collections Library at the University of New Hampshire to thoroughly examine Betty Hill's iconic dress. Ben, a former special agent with the FBI, now serves as an accomplished television producer. As the trustee of Betty Hill's estate and her legal representative, Kathleen curated the archival collection that chronicles Betty's historical legacy and, at Betty's request, donated it to UNH. I hold the exclusive authority to grant permission for scientific research on Betty's dress and to extract fabric samples from it.
During our investigation, Ben observed several intriguing stains on the dress's front lining that did not penetrate the outer fabric. Specifically, these marks seemed unlikely to be coffee stains since there were no liquid blemishes on the dress's exterior in that section, only the lining. Ben theorized that they might be blood stains and therefore requested fabric samples for DNA testing. As Betty's legal representative, I consented to take a sample from the stained area of the lining and facilitate its analysis at a scientific laboratory. I also preserved a filter disk that had been stained with Betty's blood for comparative purposes. Adhering to proper protocols, they ensured that gloves and clean shears were used throughout the process. We speculated whether the stained area could substantiate Betty's recollection of an incident involving a needle being inserted into her navel.
The following is a DNA analysis conducted by a scientist at MUFON's biological laboratory:
DNA case report on the historical 1961 case of the Betty and Barney Hill abduction. This was a test of a newly found stain on the Blue dress at about the waistband in the front to ascertain the origin of the DNA and it's relation to a sample of Betty Hill's blood taken at a later date.
The PCR portion of the procedure was
accomplished on the Stratagene RoboCycler Temperature Cycler and a Biorad Thermo mixer. The E gel electrophoresis system with
illuminator and using 2% agarose E gels. All Reagent kits purchased from Thermofisher Scientific of
Carlsbad California. The kit was a new procedural kit Thermo Scientific Phusion Human specimen Direct
PCR that has been found to be effective in procuring DNA from older and minimal samples. All
procedures were done while wearing a head covering, face mask, gloves and lab coat freshly washed
and bleached and used for DNA procedures only. All handling of DNA and any tools used therein were
sterilized and handled via proper laboratory norms for DNA processing. All vials, pipette tips etc.
were sterile as well. A
2x3mm punch sample was taken of the stained cloth and processed in a reagent
solution provided by BlueStar Hemoglobin test kit for the presence of blood. The test was found to
be negative for blood.
The test was performed twice to insure the testing cassettes were giving a uniform reading. One line at the C position is an indicator that the reagent used is working as expected. A second line at the T position would have indicated the presence of blood. Therefore, the tests were both negative. Whether it was due to the age of the sample (1961) or it not being a blood stain but rather a sweat or other bodily fluid stain the indication was a definite negative for blood.
The Thermo Scientific Phusion Human specimen Direct PCR Kit can be used with several types of samples the negative blood test was not a prohibitor of its use and the protocol followed from the kits instruction sheet was as follows:
Two 3mm punches were taken from the material in two
different areas of the sample cloth sample and blood sample on paper using tissues punches purchased from Ted Pella Co. they
were placed in separate 1.5 ml vials, one for the dress stain and another for the blood on
paper, containing 50 ul dilution buffer, 1.5 ul DNA-release additive (provided with kit) and 250ul TE
(Tris /EDTA) pH 8. purchased from Thermo scientific. The samples were rotated to loosen stain
material and blood then the cloth and paper was removed using a sterile instrument by pulling them up the
sides of the vial and
disposing of them in the proper manner. Both vials were mixed by vortex and
spinning them down in a centrifuge at 10,000 rpm. The vials containing the samples were then incubated
at 98 degrees Celsius for two min., spun down again and 0.5 ul of the supernatant used as a template
for a 20ul PCR reaction. The primers for the PCR reaction were as follows:
Primer #1 (24-mer)
5'- AGCCCTTGGGGASTTGAATTGCTG – 3'
Melting point: 73.5 degrees C
Primer#2 (27 mer)
5'- GCACTCCAGAGGACAGCRGTGTCAATA - 3'
Melting point 72.2 degrees C(R=A), 75.3 degrees C (R=G)
Both primers included in Thermo Scientific Phusion Human specimen Direct PCR
Kit
Pipetting instructions for PCR reaction are as follows:
H2O 20ul
2xPhusion human specimen PCR buffer 10ul
Universal control primer mix 0.4 ul
Phusion Human specimen 0.4 ul
DNA Polymerase
Dilution protocol: 0.5 ul
Cycling program for the Robocycler:
98 degree C 5min for 1 cycle (Lysis of cells)
98 degrees C 1s
Denaturation
72 degrees C 15s Annealing/Extension 45 cycles each in succession.
72 degrees C 1min Final Extension 1 cycle
4.0 degrees C Hold
In conclusion the unknown stain and the known blood sample for Betty Hill are a match. Although the stain read negative for human blood and could have been a sweat stain or so degraded for hemoglobin it did not register on the blood cassette used for the blood test, it must be concluded that this stain came from body fluids of the wearer of the dress (Betty Hill)." *Note: sweat stains were not found elsewhere on the dress. (end report)
XRF Analyses on Three Dress Samples
A seasoned chemistry professor with thirty-five years of experience, along with his esteemed colleague (names withheld), an associate professor who boasts thirty years at the major university oversaw a chemistry major tasked with analyzing three samples from Betty Hill's dress. The fabric samples from the exterior of Betty Hill's dress were sent to them in separate plastic bags back in 1992. On the morning of February 7, 2020, he reached out to Kathleen Marden, informing her that one of his students would perform X-ray fluorescence analysis to identify heavier elements present on Betty's dress. The student had a background in carrying out scans on various soft pastels sourced from China. (To avoid the risk of mockery, the identifying information regarding the university, instructors, and students involved in this analysis has been intentionally left out.)
The professor of chemistry stated the following:
"The student was told nothing until he completed the scans. He used an instrument manufactured by Oxford Instruments (Compact Eco) which is a benchscale instrument.
There are models also used in the field by scientists.
The student wrote down the details for each sample in the plastic holders which you sent me.
Waistline:
Ti, Mn and Ba were detected (0.5% level can be detected for calcium and heavier elements).
2nd sample: Mn, Ga, Ge and La.
3rd sample: Ti, Mn, Fe, Se, Te and Rh.
The print dress has dyes and mordants holding the dye to the fabric as would be expected. Aluminum salts are often used, and it was not observed. Iron salts can and we saw it on only one sample.
The associate professor and I were amazed at Te and Rh showing up on / in fabric.
Selenium has been used in 1950s era oil-based paints and we have scanned a number of such paints from a company in Cincinnati that was later purchased by Crayola. We found selenium (very poisonous element) in one such paint. The rare and expensive Rh was observed. It would never be expected in clothing. The same with Te.
I would suggest that more extensive scans be done by a chemistry professor in a graduate chemistry dept. They can corroborate our cursory findings and perhaps detect other elements.” (end report)
Rare Elements Found on Betty Hill's Dress
Tellurium is a very rare element that averages only three parts per billion in the Earth's upper crust. It is more prevalent in space. It shows a close association with gold and may be present in ore bodies of most gold deposit types at levels of tens to hundreds of parts per million. However, orebodies likely have concentrations of 0.1 to 1.0 parts per million. Although it is rare in the Earth's crust, it is far more prevalent in the universe. (Tellurium-USGS Publications, pubs.usgs.gov/publication/pp1802Rpse-info.de/en/element/Te)
The most valuable precious metal on the planet, Rhodium is also the rarest element. It makes up only 0.0002 parts per million of the earth's crust. It is resistant to corrosion with a high melting point and is an excellent catalyst. It is durable and resistant to heat, wear, and corrosion. " (www.theassay.com, http://science.howstuffworks.com)
